| Congresso Brasileiro de Microbiologia 2023 | Resumo: 590-1 | ||||
Resumo:The prevalence and abundance of Acidobacteriota, a bacterial phylum, have raised concerns regarding their ecological function and metabolic activity within the environment. While some studies have reported their potential to interact with plants and participate in biogeochemical cycles, their specific role in these contexts remains largely unexplored. To address this knowledge gap, we conducted a comprehensive genomic analysis encompassing 758 metagenome-assembled genomes (MAGs) from the Genomes from Earth’s Microbiomes catalog and 121 RefSeq genomes of Acidobacteriota. Our analysis encompassed a diverse dataset from various environments, including terrestrial and aquatic environments, plant host-associated systems, and engineered environments, covering MAGs from all continents. We performed alignment of MAGs' protein sequences to identify genes related to plant growth-promoting traits (PGPTs) carried out using a combination of BLASTP and HMMER tools in the PGPT-Pred database from PLaBAse and profiled metabolic, biogeochemical traits, and functional networks in the Acidobacteriota MAGs. Our analysis yielded significant findings, particularly regarding the presence of PGPT genes within several families of Acidobacteriota, including Acidobacteriaceae, Bryobacteraceae, Koribacteraceae, and Pyrinomonadaceae. These families exhibited a high frequency of PGPT genes, suggesting their potential interactions with plants. The PGPTs encompassed genes involved in various beneficial processes for plant growth, such as nitrogen fixation, phosphorus solubilization, exopolysaccharide, and siderophore production, as well as plant growth hormone production. Notably, the expression of these genes was observed to be actively transcribed across diverse environments, including in the rhizosphere and bulk soil microbial community. Furthermore, we identified numerous carbohydrate-active enzymes and peptidases responsible for the degradation of plant polymers, thus highlighting their involvement in carbon cycling. Additionally, we elucidated the of role Acidobacteriota in nitrogen, sulfur, and trace metal cycling, including the functional potential for biological nitrogen fixation in Acidobacteriota and providing valuable insights into the distributional aspects of this phylum. By highlighting the distinct ecological roles of each taxonomic group within Acidobacteriota, our research offers valuable insights for future investigations in this field. This expanded knowledge paves the way for further studies on their ecological significance, metabolic activities, and potential applications in plant growth promotion and biogeochemical cycling. Palavras-chave: Ecological roles, metagenomics, plant growth-promoting bacteria, soil bacteria Agência de fomento:CNPq, FAPEMIG, CAPES | |||||